In 2001, the Delaware & Hudson and Genesee & Wyoming jointly purchased the South Buffalo Railway (an industrial shortline just south of Buffalo that both the D&H and G&W had been attempting to purchase for a while; when the SBR’s current owners indicated that they’d be interested in selling the D&H & G&W agreed that a joint purchase would work out better than an expensive bidding war would have been) and started operating it as a 50/50 joint line.

The South Buffalo used to roster a wide variety of Alco switchers, but by 2001 those unit were long gone; the EMD switchers that were left went to the G&W, and power is supplied from the D&H & G&W’s Buffalo & Pittsburgh Railroad. Some of the locomotives that now operate on the line have been painted for the SBR; the ones supplied by the B&P are in the regular G&W paint scheme, but are lettered and logoed (a cute drawing of a buffalo in the middle of the G&W medallion) for the SB, and the ones supplied by the D&H are painted in the regular yellow & black SBR paint scheme.

As of 2025, the South Buffalo operates on its 12 route miles of track, plus it does switching for the B&P along the line from Lackawanna to Orchard Park, NY.